ABSTRACT
Imatinib mesylate (IM) is used to treat chronic myeloid leukemia (CML) because it selectively inhibits tyrosine kinase, which is a hallmark of CML oncogenesis. Recent studies have shown that IM inhibits the growth of several non-malignant hematopoietic and fibroblast cells from bone marrow (BM). The aim of the present study was to evaluate the effects of IM on stromal and hematopoietic progenitor cells, specifically in the colony-forming units of granulocyte/macrophage (CFU-GM), using BM cultures from 108 1.5- to 2-month-old healthy Swiss mice. The results showed that low concentrations of IM (1.25 µM) reduced the growth of CFU-GM in clonogenic assays. In culture assays with stromal cells, fibroblast proliferation and α-SMA expression by immunocytochemistry analysis were also reduced in a concentration-dependent manner, with a survival rate of approximately 50% with a dose of 2.5 µM. Cell viability and morphology were analyzed using MTT and staining with acrydine orange/ethidium bromide. Most cells were found to be viable after treatment with 5 µM IM, although there was gradual growth inhibition of fibroblastic cells while the number of round cells (macrophage-like cells) increased. At higher concentrations (15 µM), the majority of cells were apoptotic and cell growth ceased completely. Oil red staining revealed the presence of adipocytes only in untreated cells (control). Cell cycle analysis of stromal cells by flow cytometry showed a blockade at the G0/G1 phases in groups treated with 5-15 µM. These results suggest that IM differentially inhibits the survival of different types of BM cells since toxic effects were achieved.
Subject(s)
Animals , Male , Mice , Antineoplastic Agents/pharmacology , Benzamides/pharmacology , Hematopoietic Stem Cells/drug effects , Mesenchymal Stem Cells/drug effects , Piperazines/pharmacology , Pyrimidines/pharmacology , Cell Proliferation , Cell Survival , Cells, Cultured , Colony-Forming Units Assay , Fibroblasts , Hematopoietic Stem Cells/cytology , Immunohistochemistry , Mesenchymal Stem Cells/cytologyABSTRACT
Relato de caso de paciente de 16 anos de idade, com diagnóstico de infecçäo cérvico vaginal por Papilomavírus Humano (HPV), sugerido inicialmente através de citologia oncótica pela presença evidente de coilócitos, critério patognomônico para HPV e posteriormente comprovado por Captura Híbrida a presença de HPV de alto potencial oncogênico. Realizou-se também colposcopia, onde se observaram lesöes em colo e vulva características do mesmo agente, contudo o estudo histopatológico demonstrou alteraçöes displásicas, sem entretanto caracterizar presença de qualquer agente específico
Subject(s)
Humans , Female , Adolescent , Colposcopy , Papillomaviridae , Parvoviridae Infections , Vaginal Smears , Cervix Uteri , Cytological Techniques , Histological Techniques , VulvaABSTRACT
O objetivo deste trabalho foi realizar um levantamento, em relaçäo ao perfil metodológico do exame do líquido pleural no Estado de Santa Catarina, já que existem numerosos métodos para o estudo do líquido pleural. Para avaliar a metodologia aplicada nos laboratórios do Estado, foram enviados questionários para os laboratórios das maiores cidades, quanto as análises citológicas, bioquímicas e microbiológicas da amostra de líquido pleural näo há uma homogeneidade nos protocolos entre os laboratórios para a maioria das análises. Isso se deve provavelmente a uma adaptaçäo da rotina de acordo com as condiçöes do serviço e do paciente atendido
Subject(s)
Humans , Pleural Diseases/microbiology , Body Fluids/cytology , Pleural Effusion , Biological Specimen Banks , Clinical Laboratory TechniquesABSTRACT
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4 percent casein as compared to 20 percent casein in the control diet. When the experimental group had attained a 20 percent loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5 percent) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when compared to the control group. These results suggest an association between fluctuations in the composition of the hematopoietic microenvironment and altered hematopoiesis observed in undernourished mice
Subject(s)
Animals , Male , Mice , Bone Marrow Cells/chemistry , Extracellular Matrix/chemistry , Fibronectins/metabolism , Laminin/metabolism , Protein-Energy Malnutrition/metabolism , Blotting, Western , Case-Control Studies , Electrophoresis, Polyacrylamide Gel , Fibronectins/analysis , Glycoproteins/analysis , Hematopoiesis, Extramedullary/physiology , Laminin/analysisABSTRACT
Em 109 escolares de 8 a 12 anos de idade provenientes de zona periferica da cidade de Campinas-SP,encontrou-se uma prevalencia de parasitos intestinais de 42,2%, a media de concentraçao da hemoglobina sanguinea foi de 13,57g/dl 0,98 e concentraçao serica de ferritina de 43,67 ng/ml 21,34. No estudocomparativo entre a populaçao infectada e nao infectada por parasitas intestinais nao houve diferenças estaristicamente significativas para dosagem de hemoglobina e ferritina serica.